Target Protein Information

• Target Protein: c-Kit/CD117

• Concentration: 1mg/ml

• Form: Liquid

• Host: Rabbit

• Clonality: Polyclonal

• Isotype: IgG

Applications

• IHC-P: 1:100-500

• IHC-F: 1:100-500

• Flow-Cyt: 1ug/Test

• ICC/IF: 1:100-500

• IF: 1:100-500

Reactivity

• Human

• Mouse

• Rat

• (Predicted: Dog, Horse)

Additional Information

• Predicted MW: 105 kDa

• Entrez Gene: 3815

• Swiss Prot: P10721

• Source: KLH conjugated synthetic peptide derived from human c-Kit/CD117: 301-400/976

• Purification: Affinity purified by Protein A

Storage and Shipping

• Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• Shipping: Shipped at 4℃.

Background

c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto-oncogene. It regulates various biological responses such as cell proliferation, apoptosis, chemotaxis, and adhesion. Ligand binding to its extracellular domain triggers autophosphorylation on several tyrosine residues within its cytoplasmic domain, leading to its activation. Mutations in c-Kit have been implicated in tumor growth and progression in multiple cancers, including mast cell diseases, gastrointestinal stromal tumors, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 721 of c-Kit facilitates the binding and activation of PI3 kinase.