产品类型 内参抗体、重组兔单抗、核蛋白内参抗体

英文别名 Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen; PCNA_HUMAN.

产品应用 WB=1:1000-10000, IHC-P=1:200-1000, IHC-F=1:200-1000, Flow-Cyt=1ug/Test, ICC/IF=1:100-500, IF=1:200-1000

Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.

交叉反应 Human, Mouse, Rat

抗体来源 Rabbit

免疫原 Recombinant human PCNA protein

亚型 IgG

注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

数据库链接

Entrez Gene : 373984 Chicken

Entrez Gene : 515499 Cow

Entrez Gene : 37290 Fruit Fly

Entrez Gene : 5111 Human

Entrez Gene : 18538 Mouse

Entrez Gene : 25737 Rat

Entrez Gene : 30678 Zebrafish

Omim : 176740 Human

背景资料

Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

功能

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232).
Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902).
Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

保存条件

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.